Achievements

Division > Plant Biotechnology and Cytogenetics> Achievements

  • Obtained a Patent (a team of four researchers) on “A process for in vitro plantlet production of the bamboo, Oxytenanthera stocksii” The patent was granted on 31.08.2006 for a term of 20 years. The financial support was granted by the National Research Development Council (NRDC), New Delhi.
  • The patent titled “A simple protocol for isolation of un-degraded total RNA from Eucalyptus and Casuarina and cDNA synthesis from unpurified RNA” (Patent number 272765) was granted from 13 August 2009 for a period of 20 years. The technology was subsequently upgraded and ArborEasy® DNA isolation kit was developed for commercial production and marketing.
  • A Patent on “A process for in vitro plantlet production of the bamboo, Oxytenanthera stocksii” was granted on 31.08.2006 for a term of 20 years with the financial from National Research Development Council (NRDC), New Delhi.
  • Filed provision patent titled “A Multilocus High Resolution Melting Analysis As Diagnostic Tool For Detection Of Viral Genes” with Application number 202041018705.
  • Developed the ArborEasy® DNA isolation kit for commercial production and marketing. Trade mark for the name ‘ArborEasy’ was registered to enable branding of the DNA isolation kit.
  • Executed the license agreement with Kerafast Inc., USA for worldwide marketing of ArborEasy® DNA isolation kit. The product has been catalogued (Cat No. EIF001) in the kerafast website (https://www.kerafast.com/product/3709/ arboreasy-dna-isolation-kit).
  • Tissue culture protocols developed for mass multiplication of bamboos, teak, Eucalyptus and Acacias.
  • DNA profiling using different dominant (RAPD/ISSR/AFLP/FISSR) and co-dominant (SSR, SCAR, SNP) for species authentication, clonal discrimination, hybrid purity, genetic fidelity, diversity and population structure assessment in species targeted for tree improvement.
  • Mass clonal propagation using mini/micro cutting technology for popularization of IFGTB released clones of Casuarina and Eucalyptus.
  • Developed an online database: In silico Gene bank for adaptation to abiotic stresses.
  • Identified shoot to root ratio of sodium as a defining parameter for salt tolerance in Casuarina equisetifolia.
  • Developed composite transgenic strategy and using RNAi, identified EcHKT1:1 as a key gene regulating salt stress tolerance in Eucalyptus.
  • Based on transcriptome sequences generated from Leptocybe invasa, developed an insect-specific, multigene targeting chimeric hpRNAi for use in transgenic incorporation of tolerance to L. invasa.
  • Developed an accessory for gene delivery into live plants.
  • Barcodes for rbcL and ITS genes in Pterocarpus marsupium and P. santalinus were developed and deposited in BOLD (Barcode of life data system) and NCBI (National Centre for Biotechnology Information).
  • Whole genome assembly of Teak was generated to support conservation and breeding activities. Repetitive elements constituted 11.18 % of the genome including several thousands of simple sequence repeats (SSRs) and 615 genes encoding for proteins responsible for the durability of timber.
  • Santalum album known for its fragrant heartwood was sequenced at whole genome level, 39,800 protein coding genes identified, 574 miRNA genes and 246,522 SSRs discovered to utilize genomic tools for conservation and management efforts.
  • Whole genome sequences of two threatened rattans, Korthalsia laciniosa and Calamus brandisii was assembled and annotated to accelerate conservation of the species.
  • High throughput markers developed using target capture and re-sequencing strategy and genome-wide association analysis conducted to identify markers tagging different wood property traits in Eucalyptus mapping populations.
  • A rapid, sensitive and cost effective methodology for high throughput validation of markers in Eucalyptus was developed using High Resolution Melting (HRM) strategy.
  • A PCR based diagnostic assay was developed for detection of sandal spike disease.
  • The concept of Breeding without Breeding was applied in Eucalyptus seed orchards for early prediction of orchard gains.
  • Successfully developed a cell culture protocol for in vitro production of secondary metabolites under culture conditions from Aegle marmelos, the efficacy of which was tested against plant and human pathogens in comparison with extracts from the wild trees.
  • Developed metabolite profiles of different age genotypes of Saraca asoca  using HPTLC and GC-MS-MS.
  • Developed barcodes to differentiate Melia accessions from different locations in the country.
  • IFGTB has been designated as the DUS Centre of Melia dubia.
  • IFGTB has been identified as a Centre of Excellence by the Ministry of Tribal Affairs to work on Value addition of NTFPs.