Division > Plant Biotechnology and Cytogenetics> Achievements

  • The patent titled 'A simple protocol for isolation of un-degraded total RNA from Eucalyptus and Casuarina and cDNA synthesis from unpurified RNA' (Patent number 272765) was granted from 13 August 2009 for a period of 20 years. The technology was subsequently upgraded and ArborEasyŽ DNA isolation kit was developed for commercial production and marketing.
  • A Patent on 'A process for in vitro plantlet production of the bamboo, Oxytenanthera stocksii' was granted on 31.08.2006 for a term of 20 years with the financial from National Research Development Council (NRDC), New Delhi.
  • TC protocols developed for mass multiplication of bamboos, teak, Eucalyptus and Acacias.
  • DNA profiling using different dominant (RAPD/ISSR/AFLP/FISSR) and co-dominant (SSR, SCAR, SNP) for species authentication, clonal discrimination, hybrid purity, genetic fidelity, diversity and population structure assessment in species targeted for tree improvement.
  • Mass clonal propagation using mini/micro cutting technology for popularization of IFGTB released clones of Casuarina and Eucalyptus.
  • A bi-functional class I chitinase gene was isolated from was C. equisetifolia and ectopically expressed in tobacco and the transgenic lines showed reduced symptoms to infection by Rhizoctonia solani.
  • A mannose binding lectin was isolated from leaves of Withania somnifera and characterized for its antipest properties against Hyblaea puera and Probergrothis sanguinolens.

  • Systems genetics strategy was used to identify major regulators of water stress response and natural variation in wood properties in Eucalyptus species.
  • Developed an online database: In silico Gene bank for adaptation to abiotic stresses
  • Identified shoot to root ratio of sodium as a defining parameter for salt tolerance in Casuarina equisetifolia
  • Developed composite transgenic strategy and using RNAi, identified EcHKT1:1 as a key gene regulating salt stress tolerance in Eucalyptus
      • Based on transcriptome sequences generated from Leptocybe invasa, developed an insect-specific, multigene targeting chimeric hpRNAi for use in transgenic incorporation of tolerance to L. invasa
      • Developed an accessory for gene delivery into live plants.
      • Barcodes for rbcL and ITS genes in Pterocarpus marsupium and P. santalinus were developed and deposited in BOLD (Barcode of life data system) and NCBI (National Centre for Biotechnology Information).
      • Whole genome assembly of Teak was generated to support conservation and breeding activities. Repetitive elements constituted 11.18 % of the genome including several thousands of simple sequence repeats (SSRs) and 615 genes encoding for proteins responsible for the durability of timber.
      • Santalum album (Sandal) known for its fragrant heartwood was sequenced at whole genome level, 39,800 protein coding genes identified, 574 miRNA genes and 246,522 SSRs discovered to utilize genomic tools for conservation and management efforts.